• Gaussian blur sigma=2, k=20 (pixel metaclusters)
  • z-score cap=3 for consensus hierarchical clustering
  • For each pixel, divide each marker by total (each pixel sums to 1)

Replicate 1

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Histogram of counts in pixel clusters

Heatmap of pixel clusters x markers

Heatmap of pixel metaclusters x markers

Replicate 2

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Histogram of counts in pixel clusters

Heatmap of pixel clusters x markers

Heatmap of pixel metaclusters x markers

Replicate 3

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Histogram of counts in pixel clusters

Heatmap of pixel clusters x markers

Heatmap of pixel metaclusters x markers

Replicate 4

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Histogram of counts in pixel clusters

Heatmap of pixel clusters x markers

Heatmap of pixel metaclusters x markers

Replicate 5

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Histogram of counts in pixel clusters

Heatmap of pixel clusters x markers

Heatmap of pixel metaclusters x markers